Journal: Poultry Science

Article Title: Zinc alleviates thermal stress-induced damage to the integrity and barrier function of cultured chicken embryonic primary jejunal epithelial cells via the MAPK and PI3K/AKT/mTOR signaling pathways

doi: 10.1016/j.psj.2024.103696

Figure Lengend Snippet: Information about primary antibodies.

Article Snippet: PI3K , 1:2000 , bsm-33219M , Bioss , China.

Techniques:

Journal: Endocrine, Metabolic & Immune Disorders Drug Targets

Article Title: Elucidating the Mechanism of Jisheng Shenqi Pills in the Treatment of Diabetic Kidney Disease: Network Pharmacology Combined with Experimental Verification

doi: 10.2174/0118715303339433240805045749

Figure Lengend Snippet: ( a-e ) JSP treatment improved the mRNA expression of AGE and the protein expression levels of RAGE, p-AKT/AKT and p-PI3K/PI3K in the kidney of DKD mice. ( f-h ) JSP treatment could improve the expression levels of BAX/Bcl-2 ratio, Cleaved-Caspase-3 and Bcl2 proteins in the kidney of DKD mice. ( i-k ) JSP treatment could improve the mRNA expression of IL-1β, TNF-α and MCP-1 in DKD mice kidney. ### p < 0.001 means compared with the normal control group; ## p <0.01 means compared with the normal control group; # p <0.05 indicates comparison with the normal control group; *** p <0.001 indicates that compared with the model group; ** p <0.01 indicates that compared with the model group; * p <0.05 indicates comparison with the model group.

Article Snippet: The proteins were transferred to PVDF membranes via gel electrophoresis and then blocked with a commercial blocking solution for 15 min. Next, the membranes were incubated with primary antibodies targeting RAGE (rabbit, 1:1000, Cat# YP-Ab-13666, UpingBio), PI3K (rabbit, 1:500, Cat# ET1608-70, HUABIO), p-PI3K (rabbit, 1:1000, Cat# YP-Ab-17845, UpingBio), AKT (rabbit, 1:3000, Cat# ET1609-51, HUABIO), p-AKT (rabbit, 1:2000, Cat# ET1607-73, HUABIO), BAX (rabbit, 1:5000, Cat# ER0907, HUABIO), Bcl2 (rabbit, 1:2000, Cat# ET1702-53, HUABIO), and Cleaved-Caspase-3 (rabbit, 1:1000, Cat# YP-Ab-00003, UpingBio) for 12 h at 4°C.

Techniques: Expressing, Control, Comparison

Journal: Brazilian Journal of Physical Therapy

Article Title: Can transcutaneous electrical nerve stimulation improve achilles tendon healing in rats?

doi: 10.1590/bjpt-rbf.2014.0107

Figure Lengend Snippet: Comparison of the quantity of collagen types III (%) in the tendon tissue between the TENS and Simulation groups. Data expressed in mean and standard deviation.

Article Snippet: The expression of collagen I and III, observed in the immunohistochemical reaction with the polyclonal antibodies for the collagen types I and III (Affinity Purified Anti-Collagen Type I and Type III, Rabbit Anti-Human, Rockland Inc., Gilbertsville, PA, USA), was analyzed by quantifying the area corresponding to each type of collagen.

Techniques: Comparison, Standard Deviation

Journal: Brazilian Journal of Physical Therapy

Article Title: Can transcutaneous electrical nerve stimulation improve achilles tendon healing in rats?

doi: 10.1590/bjpt-rbf.2014.0107

Figure Lengend Snippet: Measures of variables related to group, assessment, and results of Analysis of Variance (ANOVA).

Article Snippet: The expression of collagen I and III, observed in the immunohistochemical reaction with the polyclonal antibodies for the collagen types I and III (Affinity Purified Anti-Collagen Type I and Type III, Rabbit Anti-Human, Rockland Inc., Gilbertsville, PA, USA), was analyzed by quantifying the area corresponding to each type of collagen.

Techniques: Standard Deviation

Journal: Nutrients

Article Title: Arctigenin from Saussurea medusa Maxim. Targets the PI3K/AKT Pathway to Inhibit Hepatocellular Carcinoma Proliferation and Induces Apoptosis

doi: 10.3390/nu17193151

Figure Lengend Snippet: Molecular Docking Results of ARC and core target proteins: ( a ) AURKA, ( b ) CCNB1, ( c ) CCND1, ( d ) CDK1, ( e ) CDK2, ( f ) CDK4, ( g ) GSK3B, ( h ) MAP2K1, ( i ) MTOR, ( j ) PIK3CA, ( k ) PIK3R1, ( l ) RAF1, ( m ) BRAF.

Article Snippet: Simvastatin reference compound (batch number 24240515002, purity >99%) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China); Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (batch number BST17383876), GSK3B antibody (batch number 24F107311F17), mechanistic target of rapamycin (mTOR) antibody (batch number 24F114106F24), Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) antibody (batch number BOS6238BP2883), pGSK3B (Ser9) antibody (batch number 24F087204A08) were all purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); metformin reference compound (batch number L396024, purity >97%) was purchased from Shanghai Haohong Biomedical Technology Co., Ltd. (Shanghai, China); LY294002 reference compound (batch number A825051337769) was purchased from APExBIO Technology LLC. (Houston, TX, USA); pPIK3CA (Tyr317) antibody (batch number 2i44771) and pmTOR (Ser248) antibody (batch number 8g6448) were purchased from Affinity Biosciences; Cyclophosphamide reference compound (batch number H32020857) was purchased from Jiangsu Hengrui Medicine Co., Ltd. (Lianyungang, China) Model 680 microplate reader, PowerPac Basic electrophoresis unit, and electrophoresis/transfer system were obtained from Bio-Rad (Hercules, CA, USA); The FlowSight flow cytometer was purchased from Cytek ® Biosciences Inc. (Fremont, CA, USA).

Techniques:

Journal: Nutrients

Article Title: Arctigenin from Saussurea medusa Maxim. Targets the PI3K/AKT Pathway to Inhibit Hepatocellular Carcinoma Proliferation and Induces Apoptosis

doi: 10.3390/nu17193151

Figure Lengend Snippet: Molecular Dynamics Simulation Results. ( a ) RMSD profiles of protein–ligand complexes over 50 ns simulations. ( b ) Binding energy profiles (enthalpy changes) of GSK3B and PIK3CA complexes.

Article Snippet: Simvastatin reference compound (batch number 24240515002, purity >99%) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China); Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (batch number BST17383876), GSK3B antibody (batch number 24F107311F17), mechanistic target of rapamycin (mTOR) antibody (batch number 24F114106F24), Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) antibody (batch number BOS6238BP2883), pGSK3B (Ser9) antibody (batch number 24F087204A08) were all purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); metformin reference compound (batch number L396024, purity >97%) was purchased from Shanghai Haohong Biomedical Technology Co., Ltd. (Shanghai, China); LY294002 reference compound (batch number A825051337769) was purchased from APExBIO Technology LLC. (Houston, TX, USA); pPIK3CA (Tyr317) antibody (batch number 2i44771) and pmTOR (Ser248) antibody (batch number 8g6448) were purchased from Affinity Biosciences; Cyclophosphamide reference compound (batch number H32020857) was purchased from Jiangsu Hengrui Medicine Co., Ltd. (Lianyungang, China) Model 680 microplate reader, PowerPac Basic electrophoresis unit, and electrophoresis/transfer system were obtained from Bio-Rad (Hercules, CA, USA); The FlowSight flow cytometer was purchased from Cytek ® Biosciences Inc. (Fremont, CA, USA).

Techniques: Binding Assay

Journal: Nutrients

Article Title: Arctigenin from Saussurea medusa Maxim. Targets the PI3K/AKT Pathway to Inhibit Hepatocellular Carcinoma Proliferation and Induces Apoptosis

doi: 10.3390/nu17193151

Figure Lengend Snippet: The Effect of ARC on GSK3B/PIK3CA/mTOR and their Phosphorylated Protein Expression. ( a , b ) Representative Western blotting bands of mTOR, pmTOR (Ser2481), PIK3CA, pPIK3CA (Tyr317), GSK3B, pGSK3B (Ser9), and GAPDH (loading control). ( c – k ) Quantitative analysis of the relative expression or phosphorylation ratio (phosphorylated/total protein) of these proteins in different treatment groups (Con, Met, Sim, LA, MA, HA, MA + LY). Note: Compared to the control group: * p < 0.05, # p < 0.0001.

Article Snippet: Simvastatin reference compound (batch number 24240515002, purity >99%) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China); Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (batch number BST17383876), GSK3B antibody (batch number 24F107311F17), mechanistic target of rapamycin (mTOR) antibody (batch number 24F114106F24), Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) antibody (batch number BOS6238BP2883), pGSK3B (Ser9) antibody (batch number 24F087204A08) were all purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); metformin reference compound (batch number L396024, purity >97%) was purchased from Shanghai Haohong Biomedical Technology Co., Ltd. (Shanghai, China); LY294002 reference compound (batch number A825051337769) was purchased from APExBIO Technology LLC. (Houston, TX, USA); pPIK3CA (Tyr317) antibody (batch number 2i44771) and pmTOR (Ser248) antibody (batch number 8g6448) were purchased from Affinity Biosciences; Cyclophosphamide reference compound (batch number H32020857) was purchased from Jiangsu Hengrui Medicine Co., Ltd. (Lianyungang, China) Model 680 microplate reader, PowerPac Basic electrophoresis unit, and electrophoresis/transfer system were obtained from Bio-Rad (Hercules, CA, USA); The FlowSight flow cytometer was purchased from Cytek ® Biosciences Inc. (Fremont, CA, USA).

Techniques: Expressing, Western Blot, Control, Phospho-proteomics